Extended Data Fig. 5: E and NS2A dictate the host responses that differentiate vaccine and virulent YFV strains.
From: Amino acid changes in two viral proteins drive attenuation of the yellow fever 17D vaccine

a, Volcano plot of DEGs of 17D- vs Asibi-infected cells. The top 25 DEGs (by Padj) are labeled and were used for analysis in Fig. 2. DEGs are defined with a cutoff of |Log2 fold change | >2 and Padj < 0.05. b, Heatmap displaying z-score of all the DEGs in 17D- vs Asibi-infected cells. Genes were split into 8 clusters by hierarchical clustering as indicated by the row breaks. c, Mean z-score of the gene clusters in (b). d, Mean z-score of all ISGs (as identified among the DEGs in 17D- vs Asibi-infected cells). Per-gene average z-score was first calculated from 3 or 4 experimental replicates (3 or 4). Data are mean ± s.d. (c) or s.e.m. (d) of these per-gene average z-score across the indicated gene clusters (n is the number of the genes in the clusters). Padj was calculated using the Wald test followed by Benjamini-Hochberg adjustment as implemented in DEseq2.