Extended Data Fig. 7: RNA structure has a minor effect on phenotypic differences between 17D and virulent strains.
From: Amino acid changes in two viral proteins drive attenuation of the yellow fever 17D vaccine

a, The Pearson correlation coefficient squared (R²) of the average SHAPE of two experimental replicates across the genome over windows of 200 nucleotides (step size = 50 nucleotides). Dashed line indicates the R² = 0.7. Tick size at the x-axis= 2000 nucleotides. b, Additional RNA structures in NS2A of 17D and Asibi. c, Folding profiles of NS2A when different maximal paring distances were applied. d, Comparison of mScarlet-positive cells at 48 hpi (MOI = 0.05) between 17D and Asibi genomes with swapped nucleotide regions 1750-1990. Data are mean ± s.d. from 2 experiments with 3 biological samples (n = 6). Unpaired, two-tailed student t-test.