Fig. 3: RNA folding profiles of vaccine and virulent YFVs are highly similar.
From: Amino acid changes in two viral proteins drive attenuation of the yellow fever 17D vaccine

a, Median SHAPE reactivities across the genome over the centred window of 50 nucleotides. b, RNA folding profiles are shown as arc plots coloured according to base-pairing probability (see scale). Grey box labelled as i and ii highlight the low-SHAPE regions where structures formed uniquely in 17D but similarly in Asibi and Dakar. Region iii highlights the entire NS2A region. c, Scatter plots of genome-wide SHAPE reactivities averaged across two experimental replicates, showing pairwise comparison among 17D, Asibi and Dakar. R2, the square of Pearson correalation coefficient. d–i, Closer view of SHAPE reactivities and arc plot of highlighted regions (d,f,h) and representative RNA structures (e,g,i) visualized for the YFV strains examined. Region 311–389 (d,e), region 1,750–1,990 (f,g) and NS2A region (h,i). RNA structures are coloured by SHAPE reactivity (see scale).