Extended Data Fig. 1: Protein sequences in positions where 17D carries mutations are conserved among virulent strains and vaccine strains.
From: Amino acid changes in two viral proteins drive attenuation of the yellow fever 17D vaccine

a, Workflow of sequencing virulent YFV from clinical samples or passaged in rhesus macaque (YF-DakH1279). Illustration generated with figures from the NIAID NIH BIOART source. b, Highlighter plot of sequenced virulent YFVs. YF-17D was used as a reference sequence. Nucleotide differences or gaps compared to the reference sequence are highlighted in the indicated colors. The YF-17D and Asibi sequences are based on infectious clones, while all other YFV sequences correspond to the consensus sequences of the isolates. c, Dot plot showing the frequencies of detected genetic variants. Each individual dot represents a genetic variant (see Methods for variant calling). d-e, Conservation analysis of the amino acid positions where 17D deviates from Asibi. Virulent YFV (d) or vaccines (e) with complete sequences from NCBI were included for analysis.