Extended Data Fig. 3: Differences in uL3 and the PTC between the domains of life.
From: Structure of an archaeal ribosome reveals a divergent active site and hibernation factor
a, Amino acid occupancy in an alignment of uL3 sequences from representative species in bacteria, archaea, and eukaryotes84. b, Sequencing chromatogram for the rplC gene from ∆Q150-uL3 E. coli, showing the deletion of residue Q150. The chromatogram trace was broken at the site of deletion for clarity. c, Comparison of the positions of 23S rRNA nucleotides 2452 and 2504 in E. coli and archaeal ribosomes. d, RT-PCR purity assay for MS2-tagged E. coli ribosomes used in this study. Sample purity was quantified using the band intensities of the upper (MS2-tagged ribosome) and lower (WT ribosome) bands.
