Extended Data Fig. 4: Protein interaction networks and ribonuclease localization under stress conditions.
From: Aggresomes protect mRNA under stress in Escherichia coli
a-d. Relative RNA levels of representative transcripts (talB, gltL) in aggresomes versus cytosol after 30 min or 180 min of 2 mM arsenite treatment (n = 3 independent biological replicates). e, f. Protein-protein interaction (PPI) networks: e. Aggresome-associated proteins. f. Cytoplasmic proteins. g. Comparison of average local clustering coefficients between aggresome and cytoplasmic protein networks (n = 2 independent biological replicates). h-m. Subcellular distribution of ribonucleases under stress: h-j. Localization of RBN-mCherry (h), ORN-mCherry (i), and RNB-mCherry (j) during HokB toxin induction. k-m. Localization of RBN-mCherry (k), ORN-mCherry (l), and RNB-mCherry (m) during starvation. For all panels: Aggresomes marked by HslU-GFP. Right: Fluorescence intensity profiles of GFP (aggresome) and mCherry (ribonuclease) along the cellular long axis. Conditions: Ctrl (exponential phase, untreated), Ars (2 mM arsenite). Scale bar: 1 μm. Two-sided unpaired Student’s t-test used in comparison; error bars indicate SE.
