Extended Data Fig. 5: Ribonuclease surface properties and stress-induced mRNA localizatio.
From: Aggresomes protect mRNA under stress in Escherichia coli
a-c. Protein surface charge analysis (PyMOL): a. Wild-type ribonucleases (ORN, RNB, RBN). b. ME mutants: Alanine substitutions at all D/E residues within enzymatic centers. c. MO mutants: Alanine substitutions at D/E residues outside RNA-binding motifs/catalytic centers. d-e. Subcellular mRNA distribution (SIM): d. 8pepper-tagged mRNAs (nuoJ, nlpE, glpK, mukF, gmhA, dps, gppA) in untreated cells. e. Same mRNAs after 30-min 2 mM arsenite treatment. Imaging: HBC dye in imaging buffer. Scale bar: 1 μm.
