Extended Data Fig. 5: Mechanistic studies of optimal AMPs.
a–c, The SEM images of CRAB QLH-2022-637 (a), MRSA QLH-2022-718 (b) and E. coli ATCC25922 (c) treated with AMPs. SEM images of each treatment group are shown at two scale levels: 2 μm (left) and 500 μm (right). d, PCoA represents the Bray-Curtis distance between individuals in the grouping based on the FPKM value and displays the P value and F statistic. Statistical analysis was conducted using PERMANOVA. Corresponding interpreted percentages are indicated in parentheses for each principal axis (PCoA1 and PCoA2). n = 6 in each group. e, Differential gene expression analysis was shown on volcano plot to exhibition the comparison between Arkwillin-treated group and control. The x-axis represents the log2 fold change, and the y-axis shows the -log10 Padj value derived from a two-sided Wald test performed by DESeq2, with multiple testing correction using the Benjamini-Hochberg method. The vertical dashed line depicts the absolute log2foldchange > 0 threshold, and the horizontal dashed line marks the Padj ≤ 0.05 significance threshold. Genes exhibiting significant alterations are highlighted in red dot (up-regulated) or blue dot (down-regulated). Schematic in (a–c) was created with BioRender.com.
