Fig. 4: Evaluation of antimicrobial efficacy and biological properties of AMPs in vitro.

a, Proportion of AMPs with bacteriostatic effects on E. coli and S. aureus according to the diameter of the inhibition zone. The number of AMPs is indicated above each bar. b, Antibacterial activity levels of 44 m_AMPs and 14 g_AMPs against E. coli and S. aureus at high inoculum of 108 c.f.u.s ml−1. c, Cytotoxicity and haemolysis ratio of the top 20 candidate AMPs. d,e, Distribution of MIC values (d) and corresponding MIC values (e) of the top 20 candidate AMPs against E. coli, S. aureus, CRAB, MRSA and C. albicans (at standard inoculum of 105 c.f.u.s ml−1). AMPs marked with ‘#’ are the 7 leading AMPs screened for in vivo experiments. CRAB 267, CRAB QLH-2022-267; CRAB 636, CRAB QLH-2022-636; CRAB 637, CRAB QLH-2022-637; MRSA 266, MRSA QLH-2022-266; MRSA 718, MRSA QLH-2022-718.