Extended Data Fig. 3: Salivary glands change in mice immunized with Vientovirus capsid peptide.
From: Vientovirus capsid protein mimics autoantigens and contributes to autoimmunity in Sjögren’s disease
(a) Saliva flow rates were measured in BSA-, mimotope 1-, and mimotope 2-immunization groups. Dots plot with mean ± SD. n = 7 mice for the BSA group, n = 6 mice for the mimotope 1 group, n = 5 for mimotope 2 group. Statistical significances were determined by Two-way ANOVA test with two-sided Tukey’s multiple comparisons test. (b) The serum IgG against SG antigens was detected by ELISA. n = 7 mice for the BSA group, n = 6 mice for the mimotope 1 group, n = 5 for mimotope 2 group. Statistical significance was determined by two-way ANOVA with two-sided Tukey’s multiple comparisons test. Dots plot with mean ± SD. (c) Immune deposits were detected by immunofluorescence staining for IgM (green) and complement C3 (red) in a representative salivary gland section from three groups. Scale bars, 250 µm. (d) Histological evaluation of lymphocyte infiltration in three groups was performed on tissue sections of salivary glands with H&E staining. Scale bars, 250 µm (left panel),100 µm (right panel).
