Extended Data Fig. 6: DSC2 and DSC3 antibodies are specific for their targets, Related to Fig. 2.
From: Epstein–Barr virus exploits desmocollin 2 as the principal epithelial cell entry receptor
a, DSC2 or DSC3 was expressed in NOK cells depleted for both DSC2/3. Sheep-anti DSC2 antibody only blocked cell-free EBV infection in DSC2 expression cells and had no effect on DSC3 expression cells. Sheep IgG as a control. b, Sheep-anti-DSC3 antibody did not block EBV infection of NOK cells expressing DSC2 only. c, Only sheep-anti-DSC3 antibody can block EBV infection of DSC3 expressing NOK cells. d, DSC2 antibody had no effect on EBV infection of Daudi cells. e, DSC2 antibody also reduced EBV cell-cell contact infection, but DSC3 antibody had no effect on EBV infection. f. The mouse monoclonal antibody #1H8G1 did not block EBV infection of NOK cells. Data are presented as mean values +/- S.D. three biological replicates, P-values were derived from two-sided t-test without adjustments, n = 3. Experiments were performed twice with similar results.
