Extended Data Fig. 7: EBV gHgL interacted with DSC2 and DSC3, Related to Fig. 3.
From: Epstein–Barr virus exploits desmocollin 2 as the principal epithelial cell entry receptor
a, Human monoclonal antibodies against gH/gL (769C2 and 769C5) or mouse monoclonal antibodies against gp350 (72A1) can immune precipitate gH-V5/gL or gp350-V5, respectively, but not immune precipitate EGFP-V5 in 293 T. 293 T cells were transiently transfected with V5-tagged EBV-gHgL and EBV-gp350 plasmids, EGFP-V5 as control. b, Anti gH/gL antibody 769C2 and gp350 antibody OT6-2 recognized gH/gL and gp350 protein in western blot, respectively. c, Anti gH/gL human antibodies co-immune precipitated gH/gL with DSC2 in NOK cells depleted for EphA2 and expressing DSC2-mCherry were co-cultured with anti-human IgG induced EGFP EBV AKATA cells. d. The direct interaction of purified His tagged EBV-gH/gL and Fc-tagged DSC2 extracellular domain (EC1-5) were shown by ELISA, but not with His tagged gp350, n = 6. e, The binding affinity of purified EBV-gH/gL and DSC2 were analysed by ELISA. EC50 as the binding affinity constant value. OD450max indicates maximum absorbance. Data are presented as mean values +/- S.D. (d, e) six biological replicates in two independent experiments, (c) P-values were derived from two-sided t-test without adjustments. Experiments were performed twice with similar results.
