Extended Data Fig. 1: Identification of a mitochondrial gene, PET309, involved in C. auris fluconazole susceptibility using the PB-based mutagenesis system.
From: Candida auris vacuolar calcium pump mediates fluconazole efflux and resistance evolution
a Heatmap of the Pearson correlation coefficients (R) calculated across biological triplicates within and between screening conditions. b Volcano plots of the FSI values for PB transposition mutants grown in YPD containing 30, 60, or 120 μg/mL fluconazole compared with the same mutants grown in drug-free YPD. The PB-mutated genes represented by mutants that show statistically significant fold increases (FSI ≥ 1 and p < 0.05) or decreases (FSI ≤ 1 and p < 0.05) after drug treatment are indicated in red or blue, respectively. Three genes known to be associated with fluconazole susceptibility are highlighted in purple. The number of mutants that meet our selection criteria ( | FSI | ≥ 1, p < 0.05) is indicated on the top of each volcano plot. Statistical significance was tested using the Wald test with the Benjamini-Hochberg correction. c Spotting of the indicated strains on YNB plates supplemented with 2% glucose or 2% glycerol. Cells were grown overnight in YPD, washed twice with PBS, and resuspended in PBS at a final concentration of 1 × 107 cells/mL. Subsequently, 3 μL was spotted in serial 10-fold dilutions. Images were taken after 48 h of growth at 30 °C. The experiment was repeated twice with similar results. d Dose-response assays for the indicated strains were performed as described in Fig. 1f. Fluconazole was applied as 2-fold serail dilutions ranging from 0.25 to 256 µg/mL. After incubation at 30 °C for 48 h, growth was measured and normalized to the no-drug control. Averaged technical triplicates are represented by the color scale. The experiment was repeated twice with similar results.
