Extended Data Fig. 2: Characterization of the screening strain, CauW156.
From: Candida auris vacuolar calcium pump mediates fluconazole efflux and resistance evolution
a Genotype description of CauW156. Arrows indicate primers used for genotyping. b PCR-based genotyping of CauW156 using primers shown in (a). WT and CauW08 cells were included as controls. The experiment was repeated twice with similar results. c Dose-response assays for the indicated strains were performed as described in Fig. 1f. Fluconazole was applied as 2-fold serial dilutions ranging from 0.25 to 256 µg/mL. After incubation at 30 °C for 48 h, growth was measured and normalized to the no-drug control. Averaged technical triplicates are represented by the color scale. The experiment was repeated twice with similar results. d Dose-response assays for the indicated strains were performed as described in Fig. 1f. Fluconazole was applied as 2-fold serial dilutions ranging from 0.25 to 256 µg/mL. After incubation at 30 °C for 48 h, growth was measured and normalized to the no-drug control. Averaged technical triplicates are represented by the color scale. The experiment was repeated twice with similar results.
