Extended data Fig. 7: Lipophilic P-G3 NPs retains the effects of P-G3.
From: Selective targeting of visceral adiposity by polycation nanomedicine
a, NMR spectrum of P-G3 and P-G3-Chol(5). b, The internalization of Cy5-labelled NPs into early endosome. Confocal images of Cy5-labelled NPs with early endosome marker in mature C3H10T1/2 adipocytes after 15 min or 1 hr of Cy5-NPs treatment. Representative data repeated twice with similar results. c, C3H10T1/2 preadipocytes were treated with 10 μg/ml P-G3 or NPs since the induction of differentiation Day 0, and cells were harvested on Day 4 to measure adipogenic genes by qPCR. Data were represented as mean ± s.e.m. (n = 4, 4). Statistical significance was calculated via 2-tailed Student’s t-test (treatment group vs vehicle group). d, e, 200 μg Cy5-labled NPs or Cy5-labelled P-G3 were i.p. injected into mice in Fig. 6c; d, signal intensity quantification of tissue distribution and at 72-hr post-injection by IVIS (PBS group n = 1, P-G3 group n = 2, NPs group n = 3); e, colocalization of Cy5-labelled NPs with DAPI and Caveolin-1 in frozen sections of eWAT. f, Macrophage-related gene expression in the eWAT after NP treatment. Data were represented as mean ± s.e.m. (n = 8, 8). Statistical significance was calculated via 2-tailed Student’s t-test. g, Body composition change of NPs-treated DIO mice during 24-hr fasting and 24-hr refeeding. Data were represented as mean ± s.e.m. (n = 8, 8). h, WB analysis of mTOR signalling pathway in eWAT after NPs treatment and the quantification. Data were represented as mean ± s.e.m. (n = 4, 5). Statistical significance was calculated via 2-tailed Student’s t-test.
