Extended Data Fig. 8: Representative images of an HS-AFM video showing ACE2 association and dissociation from an S trimer \({\mathbf{S}}_{\mathbf{Wuhan}}^{\mathbf{f-}}\) already interacting with two other ACE2.
From: Modulation of SARS-CoV-2 spike binding to ACE2 through conformational selection
Unbound ACE2 are indicated by cyan arrowheads, bound ACE2 by green arrowheads, the stalk region by a white arrowhead, and open RBDs by yellow arrowheads. Timestamps are displayed in the top right corner of each image. The relative orientation of the ACE2 to the RBD causes the RBD to appear brighter compared to the other RBDs without ACE2 binding. On the left, a simulated AFM image is generated from the cryo-EM structure of three ACE2 bound to one S trimer (PDB: 7kms) to illustrate the relative positioning of ACE2 and RBD in the experimental observation.
