Extended Data Fig. 4: Myeloid Anxa1 deficiency exacerbates SAP. | Nature Nanotechnology

Extended Data Fig. 4: Myeloid Anxa1 deficiency exacerbates SAP.

From: Annexin A1 mRNA-loaded liposomes alleviate acute pancreatitis by suppressing STING pathway and promoting efferocytosis in macrophages

Extended Data Fig. 4

(a) Pancreas histology scores in Anxa1f/f and Anxa1CKO mice that were saline-treated (NaCl group) or L-arginine-treated (SAP group). The proportion of apoptotic (b) and necrotic cells (c) in pancreas tissues. Pancreas IFN-β (d), TNF-α (e), IL-6 (f), and MCP-1 (g) in Anxa1f/f and Anxa1CKO NaCl or SAP mice. (h) (a) Representative immunoblot analysis of TLR4, STING, p-TBK1, TBK1, p-P65, P65, and NLRP3 in pancreas tissues from Anxa1f/f SAP and Anxa1CKO SAP mice. HA-M@Lip@Anxa1 NPs treatment of SAP mice induced with injection of L-arginine. The pancreas pathology score (i), phagocytic index (j), pancreas IFN-β (k), TNF-α (l), IL-6 (m), and MCP-1 (n) in phosphate buffer saline (PBS) and HA-M@Lip@Anxa1 NPs-treated saline control (Con) and SAP mice. (o) Immunoblot analysis of TLR4, STING, p-TBK1, TBK1, p-P65, P65, and NLRP3 in pancreas tissues of SAP mice after receiving PBS or HA-M@Lip@Anxa1 NPs treatment. Data are presented as means ± SD of n = 5 biologically independent samples (a–g, i–n). P values are determined by unpaired two-tailed Student’s t-test (b–g, j–n) or one-way ANOVA with Tukey’s post-hoc test (a and i).

Source data

Back to article page