Extended Data Fig. 1: Myeloid Anxa1 expression in the mouse pancreas during AP.
(a) RNA-seq of pancreas in AP mice with different time points. (b) Anxa1 mRNA expression in the pancreas in AP mice at different time points. * indicates compared with control; # indicates compared with 24 h; NS, not significant as compared with control. (c) Monocyte abundance of pancreas in AP mice with different time points. * indicates compared with control; (d) Macrophage abundance in the pancreas in AP mice at different time points. * indicates compared with control; NS, not significant as compared with control. (e) Correlation between monocyte/macrophage abundance and the expression of Anxa1. (f) UMAP plot showing 6 immune cell clusters in the pancreas of AP mice. (g) Fraction of 6 immune cell clusters in each sample. (h) Dot plot depicting percent expression and average expression of Anxa1 in CER and control groups. (i) Flow cytometry analysis of Anxa1 expression in CD45+F4/80+ cells from the mouse pancreas during AP and recovery. *P < 0.01 as compared with 0 h; #P < 0.05 as compared with 12 h; NS, not significant as compared with 0 h. (j) Flow cytometry analysis of Anxa1 expression in CD45+F4/80+ and CD45+F4/80− cells from CER mice. NS, not significant as compared with control. Data are presented as means ± SD of n = 3 (b–d), n = 5 (i), or n = 10 (j) biologically independent samples per group. Data are presented as means ± SD of n ≥ 3 (b–d), n = 5 (i), or n = 10 (j) biologically independent samples per group. P values were determined by one-way ANOVA with Dunnett’s post-hoc test (b–d), Spearman’s correlation (e), unpaired two-tailed Student’s t-test (j) or one-way ANOVA with Tukey’s post-hoc test (i). Illustrations in a created with Figdraw.com.
