Extended Data Fig. 6: Experimental comparisons of different SOFI methods, SACD, and Sparse-SACD.
From: Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging
(a) Microtubule filaments in a COS-7 cell labelled with QD525 imaged by the wide-field microscopy (left) and 2D-SIM (right) (c.f., Fig. 1). (b, c) SACD without (w/o) the linearization step (b) and the autocorrelation step (c). As pointed out by the white arrows, SACD without the autocorrelation step failed to resolve fine structures. (d, e) Reconstruction results using 20 frames (d) and 1000 frames (e). (f, g) Reconstruction results of live COS-7 cell labelled with Skylan-S-TOM20 imaged at 37 °C imaged by SD-confocal (c.f., Fig. 4d) using 20 frames at time points 0 min 0 s (f) and 10 min 40 s (g). Details in Supplementary Note 9. Experiments were repeated ten times independently with similar results. Scale bars: 1 μm.
