Extended Data Fig. 7: Representative 3D microtubule network reconstructed by SACD.
From: Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging
(a) Color-coded three-dimensional distributions of microtubule filaments in a COS-7 cell labelled with QD525 under SD-confocal (top left) and SACD (top right) images. The bottom panels show the xy slices (at 0 μm of z axial position) of SD-confocal (left) and SACD (right). (b) Corresponding vertical sections of SD-confocal (top) and SACD (bottom). (c) Axial resolution comparison using the enlarged regions of the white boxes indicated in (b). From left to right: cross-sections of SD-confocal, SOFI (20 frames), and SACD (20 frames) results, and the intensity profiles indicated by red arrows are displayed on the right. Experiments were repeated ten times independently with similar results. Scale bars: (a) 5 μm; (a inset, b) 2 μm; (c) 500 nm.
