Fig. 3: Cortex-wide functional imaging of the neural activity in mice in vivo during epilepsy.

a–e, Cortex-wide functional imaging of neural activity during epilepsy in Ai148D/Rasgrf mice. a, Spatiotemporal map of the successive recruitment of Rasgrf-expressing neurons within a 4.8 × 6.4 mm FOV (recorded at 1.67 Hz). Neuron segmentations are colour-coded according to onset time in the selected seizure event, as noted by the arrow in b. b, Calcium signals corresponding to the neuron segmentations in a. c, Neurovascular coupling in seizures. Vascular dilations are measured along the yellow lines shown in a, and the calcium signals are the averages of the fluorescence signals inside the yellow dashed boxes in a. d, Colour-coded seizure-spreading event in a 4.8 × 3.2 mm area (as denoted by the orange dashed box in a), recorded at 3.3 Hz. e, Intensity changes of pixels versus time along line 1 in d. f–j, Cortex-wide functional imaging of neural activity during epilepsy in Ai162/PV mice. f, Spatiotemporal map of successive recruitment of parvalbumin-expressing (PV-expressing) neurons within a 4.8 × 4.8 mm FOV (at 2.8 Hz). Neuron segmentation results are colour-coded according to the onset time in selected seizure events, as denoted by the arrows in g. g, Calcium signals corresponding to the neuron segmentations in f. The upper and lower panels correspond to neural activities in the left and right brain hemispheres, respectively. h, Neurovascular coupling in seizures. Vascular dilations are measured along the yellow lines shown in f, and the calcium signals are the averages of the fluorescence signals inside the yellow dashed boxes in f. i, Colour-coded seizure-spreading events in the 4.8 × 4.8 mm imaging area. j, Intensity changes of pixels versus along line 1 in i.