Fig. 5: The 1S2F scheme speeds up the RA-WiFi mesoscope. | Nature Photonics

Fig. 5: The 1S2F scheme speeds up the RA-WiFi mesoscope.

From: Random-access wide-field mesoscopy for centimetre-scale imaging of biodynamics with subcellular resolution

Fig. 5

a, Cortex-wide imaging of a Ai148D/Rasgrf mouse brain with a 6.4 × 6.4 mm FOV. The two selected ROIs for 1S2F imaging are denoted by the boxes. b, Superimposed image of neuron segmentations, based on the subsequent 1S1F imaging of ROIs i and ii. c, Neuron segmentations based on 1S2F imaging. d, Calcium signals extracted via 1S2F imaging. The signals in ROIs i and ii can be distinguished. e, Representative neural activities in ROIs i and ii. f, The neuron-to-neuron CCs after hierarchical clustering and sorting. g, Cortex-wide imaging of a B6 mouse brain with FITC-labelled blood plasma in 4.8 × 4.8 mm FOV. The two selected ROIs are denoted by the boxes. h, Colour-coded superimposed morphologies of blood vessels obtained via the subsequent 1S1F imaging of ROIs i and ii. i, Morphology of the blood vessels obtained via 1S2F imaging. j, Relative dilation of blood vessels 1 and 2 along the coloured lines in i. A moving time average filter with a window of ~1 s is applied (solid lines).

Back to article page