Extended Data Fig. 6: Optical setup for 3D-MP- SIM based on fast pure phase HS-SLM. | Nature Photonics

Extended Data Fig. 6: Optical setup for 3D-MP- SIM based on fast pure phase HS-SLM.

From: Fast, three-dimensional, live-cell super-resolution imaging with multiplane structured illumination microscopy

Extended Data Fig. 6

a, The original illumination optical path based on the piezo stage and the new illumination optical path based on pure phase HS-SLM are integrated with RM, with a detailed description in the Methods section. AOTF1-2, acoustic-optical tunable filter; SLM1-2, spatial light modulator; HS-SLM, pure phase high-speed spatial light modulator; HWP1-2, half-wave plate; PBS1-2, polarizing beamsplitter; L1-L11, lens; M1-M4, mirror; HS-LCVR, high-speed liquid crystal variable retarder; QWP, quarter-wave plate; DM, dichroic mirror; OBJ, objective; FW, filter wheel; ISP, image splitting prism; RM, rotatable mirror. b, Photograph of the 3D-MP-SIM microscope that integrated with pure phase HS-SLM for faster axial phase shifting.

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