Extended Data Fig. 7: Dynamics of ER in living cells visualized by 3D-MP- SIM integrated with pure phase HS-SLM. | Nature Photonics

Extended Data Fig. 7: Dynamics of ER in living cells visualized by 3D-MP- SIM integrated with pure phase HS-SLM.

From: Fast, three-dimensional, live-cell super-resolution imaging with multiplane structured illumination microscopy

Extended Data Fig. 7

a, MIP of the ER by expressing KDEL-mStayGold in the live COS-7 cells imaged with 3D-MP-SIM. b, Magnified x-y and the corresponding x-z cross-sections along the extension tubular ER of the boxed regions in a at the indicated time points. Arrowheads highlight the ER dynamics, specifically illustrating how a tubular ER rapidly crosses another tubular ER below it at 2.51 s and undergoes rapid contraction at 5.45 s. Scale bars: 2 µm (a); 1 µm (b). All cross-section slices are 30 nm thick.

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