Extended Data Fig. 2: Resolution quantification from beads and microtubules using FWHW.
a, The FWHMs were measured on 150 beads from 4 independent experiments for each imaging mode. WF: 244.38 ± 10.86 nm (laterally), 499.81 ± 11.88 nm (axially); 2D-SIM: 108.14 ± 4.36 nm, 476.13 ± 12.22 nm; slice MF- SIM: 114.09 ± 5.74 nm, 497.88 ± 10.65 nm; 3D-SIM: 117.83 ± 3.53 nm, 303.32 ± 8.65 nm; 3D-MP-SIM:113.92 ± 4.94 nm, 292.26 ± 8.81 nm. b, The FWHMs were measured at 50 sub-regions from 3 independent experiments for each imaging mode. WF: 247.37 ± 9.16 nm, 565.45 ± 39.90 nm; 2D-SIM: 101.23 ± 9.16 nm, 484.57 ± 30.10 nm; slice MF-SIM: 103.63 ± 9.83 nm, 508.69 ± 37.42 nm; 3D-SIM: 110.36 ± 7.02 nm, 297.01 ± 15.64 nm; 3D-MP-SIM: 108.51 ± 8.21 nm, 282.18 ± 10.33 nm. Statistical data are presented as mean values ± s.d.
