Extended Data Fig. 3: Schematic diagram of imaging thick samples using 3D-MP-SIM by axially moving the sample.

From left to right, demonstrate moving the sample by z0 twice. The solid box represents the currently captured ‘eight-plane’ units. The dashed boxes represent regions that have been imaged previously or will be imaged in future steps. The blue arrowheads indicate the same region illuminated by the same pattern before and after movements.