Extended Data Fig. 4: Imaging thick samples using 3D-MP-SIM by axially moving the sample. | Nature Photonics

Extended Data Fig. 4: Imaging thick samples using 3D-MP-SIM by axially moving the sample.

From: Fast, three-dimensional, live-cell super-resolution imaging with multiplane structured illumination microscopy

Extended Data Fig. 4

a, MIP of immunolabelled microtubules in a fixed COS-7 cell imaged with 3D-MP-SIM; b, c. Magnified x-z cross- section (b) and y-z cross-section (c) along the dashed lines marked in a. d, MIP of the mitochondria transfected with mStayGold-OMP25 and TOM20-mStayGold in a fixed COS-7 cell imaged with 3D-MP-SIM. e, f, Magnified x-z cross-section (e) and y-z cross-section (f) along the dashed line marked in d. Scale bars: 5 µm (a, d); 2 µm (b, c, e, f). All cross-section slices are 30 nm thick.

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