Extended Data Fig. 1: Electrochemical fluorescence modulation of ATTO 655.
From: Electrochemical fluorescence modulation enables simultaneous multicolour imaging
a, Fluorescence response of ATTO 655 tagged microtubules under different buffer conditions with pulsed potential between −0.8 V and 0.4 V (top panel). In Tris buffer (gray line), fluorescence remains stable, showing minimal modulation. In low-oxygen buffer (orange line), fluorescence modulation occurs slowly with very moderate change. In low-oxygen buffer with redox mediators (potassium ferricyanide and cysteamine, blue line), nearly complete on-off fluorescence modulation is achieved. b, Demonstration of electrochemical fluorescence modulation of ATTO 655 in the x-y and x-z planes. Both planes exhibit nearly complete on-off fluorescence modulation behaviour. The images are shown with same contrast setting. c, EC spectra of ATTO 655 over 105 cycles. The EC spectra from the first (blue) and last 5 cycles (red) were normalized by subtracting their background and scaling to their maximum intensities, respectively, which shows excellent overlap. d-e, Similarly, EC spectra for Alexa 555 and mCherry over 105 cycles demonstrates high repeatability. In c-e, the potential scan rate is 4 V s-1. The frame rate is 11 ms.
