Extended Data Fig. 2: A list of EC spectra for various fluorophores.
From: Electrochemical fluorescence modulation enables simultaneous multicolour imaging
The image panels are highlighted in green, orange, and red, corresponding to the green, red and far-red spectral channels for the respective fluorophores. The EC spectra was collected from the immunostained cells with the given dye or the fluorescent protein transfected cell and imaged over a widefield epi fluorescence microscope. During the acquisition of the EC spectra, the electrochemical potential was scanned from −0.8 V to 0.4 V and back to −0.8 V at a scan rate of 500 mV s⁻¹. The EC spectra were normalized by subtracting their background and scaling to their maximum intensities. The frame rate as set at 20 ms. The imaging buffer is a low-oxygen buffer (glucose-glucose oxidase) containing cysteamine (100 mM) and ferricyanide (20 mM).
