Extended Data Fig. 3: Electrochemical colour unmixing demonstration across various z-focal planes.
From: Electrochemical fluorescence modulation enables simultaneous multicolour imaging
a, Raw fluorescence images of U-2 OS cells with ATTO 655-labeled actin and STAR RED-labelled mitochondria at different z-distances from the ITO surface, with the indicated distances of 0, 0.5, 1, 2, 3, and 4 µm, respectively. b, EC-spectra for the two dyes, green for ATTO 655, magenta for STAR RED, while the electrochemical potential was scanned from −0.8 V to 0.4 V and then back to −0.8 V with a scan rate of 0.5 V/s, the frame rate is 250 ms c, Effective separation achieved at various z-focal planes, ATTO 655-actin was labelled in green colour, STAR RED-mitochondria was labelled in magenta. Scale bar = 10 µm.
