Extended Data Fig. 4: Analysis of crosstalk as function of number of frames applied for electrochemical colour unmixing.
From: Electrochemical fluorescence modulation enables simultaneous multicolour imaging
a, Raw images showing ATTO 655-labeled actin and STAR RED-labelled mitochondria for a U-2 OS cell. b, EC-spectra for STAR RED (green line) and ATTO 655 (orange line), while the electrochemical potential was scanned from −0.8 V to 0.4 V and then back to −0.8 V with a scan rate of 0.5 V/s, the frame rate is 250 ms. c, Unmixed images obtained using 2, 4, 6, and 8 frames, demonstrating progressive improvement in separation. d, Crosstalk factor as a function of the number of frames used, showing decrease as the frame number increases from 2 to 8 frames. Scale bar = 10 µm.
