Fig. 2: Multispectral acquisition module compatible with camera-based fluorescence microscopes.

a, Optical path illustration for the multispectral imaging module. Light enters from the top left and is redirected, based on wavelength, onto eight cameras by seven dichroic mirrors. b, Photograph of the assembled multispectral imaging module. c, Transmission spectra of the seven dichroic mirrors used. d, Spectra of the eight channels arising from the spectra in c. e, USAF 1951 target imaged in the most blue channel. f, Enlarged views of the target region indicated by cyan box in e, shown for all eight channels. Green arrowheads indicate the finest resolvable group across all channels. g, Enlarged view of the target region indicated by the magenta box in f, shown for channel 1 (the green arrowhead indicates finest resolvable group in channel 1).