Fig. 3: Multispectral live-cell imaging on an SDCM.
From: Multispectral live-cell imaging with uncompromised spatiotemporal resolution
a–d, U2OS cells transfected with the ColorfulCell plasmid were incubated with LysoTracker Yellow and imaged live on an SDCM equipped with the multispectral camera system. The eight individual camera views (a) were acquired simultaneously, then processed by RLSU unmixing using a mixing matrix generated from the emission spectra of seven fluorophores (b) to generate unmixed images for the seven fluorophores (c). Colour merge of the channels in c is shown in d. e–g, Other examples of RLSU unmixing results for multispectral spinning-disk confocal imaging of U2OS cells transfected with ColorfulCell plasmid (e), or additionally incubated with SPY555-tubulin (f) or SPY555-actin (g) dyes. Scale bars, 10 μm. ER, endoplasmic reticulum; PM, plasma membrane.
