Fig. 5: Multispectral live-cell projection imaging on an OPM.
From: Multispectral live-cell imaging with uncompromised spatiotemporal resolution
a, U2OS cells transfected with the ColorfulCell plasmid were imaged by multispectral light-sheet projection imaging followed by RLSU umixing (cell boundaries in cyan). Four channels of a single frame from a video are shown (Supplementary Video 10). b, Colour merge of the channels shown in a. c, Enlarged image of the yellow box in b. d, Later frames showing Golgi fission, then fusion, in the black box in c. e, Frames from the cyan box in c showing peroxisome fission/fusion. Black arrowheads indicate peroxisomes that undergo fission or fusion within the frames shown. f, Frames displaying two-way, three-way and four-way dynamic organelle interactions within the region delineated by the red box in c. Scale bars, 10 μm (a–c) and 2 μm (d–f).
