Extended Data Fig. 4: Morphometric analysis of the nuclear shell.
From: Topological interactions drive the first fate decision in the Drosophila embryo
A, An example of a mesh reconstruction is shown for a nuclear point cloud data from a wildtype embryo during cycle 9. B, Dual/Voronoi mesh is shown for the reconstructed mesh. Facecolor indicates isoperimetric quotient (Perimeter/√Area), a measure of shape for astral domains. C, Probability density is shown for isoperimetric quotient for an example embryo (same as Extended Data Fig. 4B), with numerically associated regular polygons/circle to emphasize an intuition of the shape. D, Correlation coefficient is calculated between tilt ψ and negative strain ϵ < 0 (see Fig. 3a) after randomization. A probability distribution is plotted for the correlation coefficients upon randomization. Standard deviation of the randomization σr is shown in orange. The correlation of the experimental data is found to be ~ 2σr. E, Binscatter plots for experimental data (left) of tilt angle ψ and strain ϵ and a randomized sample of the experimental data (right) is shown. A and \(\overline{A}\) indicate domain area and its’ average. F, Scatter plots are shown for tilt angle ψ vs rescaled distance with the closest neighbour (right) and the average distance to the three closesr neighbours (left). No significant correlation is found. Pearson correlation is reported in the legend. Pooled data over n=8 WT cycle 9 embryos.
