Extended Data Fig. 3: Dynamics of nuclear shell expansion.
From: Topological interactions drive the first fate decision in the Drosophila embryo
A, A hemispherical view of the nuclear shell/boundary is shown during cycle 7-10 for wildtype (WT) and cul-5 mutants. Inflation and differences in morphology is noted. B, Average nuclear speed towards the embryo cortex is plotted for wildtype and cul-5 mutants (for different embryos), during the transition from cycle 7 to cycle 8 (left) and from cycle 8 to cycle 9 (right). Vertical dashed line=chromosome separation. C, Distance of the nuclei from the cortex is shown as a function of time for a cul-5 mutant embryo. Temporal traces (dots) and average trajectories are shown for different anterior-posterior regions (color coded shown as in the inset image). c=cycle. D, Lineage tracing of segmented and tracked nuclei are shown along their relative position in the anterior posterior axis from cycle 7 to cycle 10 for a cul-5 mutant embryo. Note that the axial spread is smaller than a WT embryo (Extended Data Fig. 1A). Different colors indicate each unique lineage trace that is, same color means it originated from the same nucleus. A=anterior, P=posterior. E, Nuclei shell area A(t) from cycle 7 to cycle 10 (A(t)–A0, with A0 = 1.2*104 μm2 for WT and A0 = 0 for cul-5 mutant) is plotted against predicted shell area from the pushing model based on nuclei number N(t) on the shell as a0N(t), where a0 = 700μm2 is the extracted domain area. Colorbar indicates time with respect to end of cycle 10. y= x line indicates the excellent agreement of the prediction while the flat dashed line indicates the saturation as the nuclear shell reaches the cortex at cycle 10.
