Extended Data Fig. 5: Physico-chemical footprints.
From: The actin cortex acts as a mechanical memory of morphology in confined migrating cells
(a) Schematic representation of the two experimental procedures used to assess pattern conditioning by migrating cells. Cells were seeded on human plasma (hp) fibronectin (FN) micropattern, then detached and fixed either (i) after 5 hours (before the time-lapse) or (ii) 24 hours (after the time-lapse) of culture on micropatterns. (b) Epifluorescence images showing human plasma fibronectin, cellular laminin and cellular FN in control conditions after t = 5 h and t = 24 h of cell culture (top to bottom). Scale bar, 50 μm. (c) Normalized laminin intensity for control (n = 62 patterns, N = 3), 5-hour conditioning (n = 67 patterns, N = 3), and 24-hour conditioning (n = 46 patterns, N = 3). (d) Normalized fibronectin intensity from control (n = 73 patterns, N = 3), 5-hour conditioning (n = 125 patterns, N = 3), and 24 hour-conditioning (n = 74 patterns, N = 3). Boxplots range from the first quartile (Q1) to the third quartile (Q3), with the median (50th percentile) indicated by a line. Whiskers extend from the box to the minimum and maximum data points within 1.5 times the interquartile range.**p < 0.01, ns = not significant (Kruskal-Wallis test).
