Extended Data Fig. 7: Myosin cortex in elongated and compacted cells.
From: The actin cortex acts as a mechanical memory of morphology in confined migrating cells
Typical equatorial focal plane obtained in enhanced-resolution microscopy showing the spatial distribution of phosphorylated myosin light chain (p-MLC) at the actin cortex in (a-b) elongated and (c-d) compacted cells. Plot profiles in (b) and (c) shows the normalized intensity of actin and p-MLC as a function of the distance from the membrane. (e) Full width at half maximum (FWHM) for myosin cortex in elongated (orange, n = 29) and compacted (purple, n = 29) cells. Boxplots range from the first quartile (Q1) to the third quartile (Q3), with the median (50th percentile) indicated by a line. Whiskers extend from the box to the minimum and maximum data points within 1.5 times the interquartile range. ****p < 0.0001 (Student’s t test). Scale bars, 10 µm.
