Extended Data Fig. 8: Effect of a nocodazole treatment on confined migration.
From: The actin cortex acts as a mechanical memory of morphology in confined migrating cells
(a) Time-lapse sequence of a compacted cell entering a bridge before (control, CTRL) and after treatment with nocodazole (orange). CTRL is DMSO for nocodazole experiments. Scale bar, 10 µm. White arrows show the direction of migration. (b) Cell shape index on bridge for CTRL (n = 21, N = 5) and nocodazole-treated cells (n = 23, N = 6). (c) Cell area on square for CTRL (n = 18, N = 6) and nocodazole-treated cells (n = 22, N = 7). (d) Successful crossing percentage for CTRL (n = 146, N = 18) and nocodazole-treated cells (n = 33, N = 8) and (e) dwell time on square for CTRL (n = 22, grey) and nocodazole-treated cells (n = 12, light orange). Boxplots range from the first quartile (Q1) to the third quartile (Q3), with the median (50th percentile) indicated by a line. Whiskers extend from the box to the minimum and maximum data points within 1.5 times the interquartile range.****p < 0.0001 and n.s. = not significant (Student’s t test).
