Extended Data Fig. 9: Thickness of the actin cortex in cells spread on squares.
From: The actin cortex acts as a mechanical memory of morphology in confined migrating cells
Enhanced-resolution confocal images of (a) a partial spreading and (b) a full spreading of epithelial cells on FN square of 1600 µm2. Cells are immunostained for F-actin with phalloidin (in green) and DNA with DAPI (in bue). Inverted images show normal and side views of the actin cytoskeleton at the apical side. The spreading rate is 50.1% for partial spreading (a) and 87.2% for full spreading (b). Scale bars, 20 µm. (c) Full width at half maximum (FWHM) represents the actin cortex thickness for partial (n = 14, N = 4) and full (n = 18, N = 4) spreading conditions. Boxplots range from the first quartile (Q1) to the third quartile (Q3), with the median (50th percentile) indicated by a line. Whiskers extend from the box to the minimum and maximum data points within 1.5 times the interquartile range. ****p < 0.0001 (Student’s t test).
