Extended Data Fig. 2: Semi-infinite narrow segments.
From: The actin cortex acts as a mechanical memory of morphology in confined migrating cells
(a) Theoretical expectation of the percentage of compacted cell on the bridge of dumbbells (W = 6 µm and L = 160 µm) with square area of 1600 µm2 versus 1D semi-infinite line of W = 6 µm and L = 500 µm. On 6 µm line, percentage of compacted cell is expressed as the percentage of time spent under the compacted morphology over a 20-hour time-lapse. (b) Time-lapse sequence of a compacted cell morphology migrating on a one-dimensional (1D) micropatterned line of W = 6 µm and L = 500 µm. Scale bar, 20 µm. (c) Representative color-coded trajectories with elongated (CSI < 0.4) and compacted (CSI > 0.4) morphologies. (d) Percentage of compacted cells on the bridge of dumbbells (W = 6 µm and L = 160 µm) with square area of 1600 µm2 (n = 56, N = 16) versus 1D semi-infinite line of W = 6 µm and L = 500 µm (n = 10, N = 2). On 6 µm line, percentage of compacted cell is expressed as the percentage of time spent under the compacted morphology over a 20-hour time-lapse.
