Extended Data Fig. 7: Cell length and velocity is affected upon inhibition of myosin or ROCK in 1D patterns with homogeneous fibronectin.
From: Single-cell migration along and against confined haptotactic gradients
a, Mean length of cells migrating on 1D patterns with homogeneous fibronectin when incubated with blebbistatin (N = 32) or Y-27632 (N = 26), compared to the control group (N = 151). Error bars are s.d. b, Mean velocity in the x-axis of cells migrating on 1D patterns with homogeneous fibronectin with treatments of blebbistatin (N = 32) and Y-27632 (N = 26), compared to the control group (N = 151). Error bars are s.d. For panels a-b, a significant difference between groups was identified using the Kruskal-Wallis test. To determine specific pairwise differences, Dunn’s multiple comparisons test was applied. The reported P values correspond to Dunn’s test. **** indicates P < 0.0001. c, First-run trajectories of cells migrating on 1D patterns with homogeneous fibronectin treated with blebbistatin (N = 32). d, First-run trajectories of cells migrating on 1D patterns with homogeneous fibronectin treated with Y-27632 (N = 26). Control data were obtained from four independent experiments. Y-27632 and blebbistatin data were obtained from three independent experiments.
