Extended Data Fig. 3: Cell deposition during migration.
From: Single-cell migration along and against confined haptotactic gradients
a, Example of protein deposition using MCF10A cells overexpressing α5 integrin-GFP. White arrow indicates the deposited tail. b, Example of membrane deposition using MCF10A cells labelled with Flipper-TR probe (Tebu-bio, SC020). White arrow indicates the deposited tail. c, Mean velocity in x for the 1st, 2nd and 3rd runs of cells migrating up and down 1D gradients. Error bars are s.d. Significant difference between groups was identified using the Kruskal-Wallis test. To determine specific pairwise differences, Dunn’s multiple comparisons test was applied. The reported P values correspond to Dunn’s test. **** indicates P < 0.0001. Immunofluorescence data were obtained from one independent experiment. Velocity data were obtained from four independent experiments. Scale bars, 10 μm (a), 5 μm (b).
