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Choose your yeast strain carefully: the RAD5 gene matters

Nature Reviews Molecular Cell Biology volume 19, pages 343–344 (2018)Cite this article

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The commonly used budding yeast strain W303 carries a mutant RAD5 gene (rad5-535), but numerous laboratories use a strain corrected for the mutation. This has resulted in different phenotypes of W303 cells in similar experiments. Here, we aim to raise awareness of the issue to ensure data reproducibility and interpretation.

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Authors and Affiliations

  1. Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK

    Menattallah Elserafy & Sherif F. El-Khamisy

  2. Center for Genomics, Helmy Institute for Medical Sciences, Zewail City of Science and Technology, Giza, Egypt

    Menattallah Elserafy & Sherif F. El-Khamisy

Authors
  1. Menattallah Elserafy
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  2. Sherif F. El-Khamisy
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Correspondence to Sherif F. El-Khamisy.

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The authors declare no competing interests.

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CommunityW303.html: http://wiki.yeastgenome.org/index.php/CommunityW303.html

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Elserafy, M., El-Khamisy, S.F. Choose your yeast strain carefully: the RAD5 gene matters. Nat Rev Mol Cell Biol 19, 343–344 (2018). https://doi.org/10.1038/s41580-018-0005-2

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