Extended Data Fig. 6: Conductances, reversal potentials, absorption spectra and kinetics of wild-type GtACR1 and mutants.
From: Crystal structure of the natural anion-conducting channelrhodopsin GtACR1
a–c, Photocurrents (a), reversal potentials (b) and absorption spectra (c) of wild-type GtACR1 and ten mutants of the retinal-binding pocket. λmax values are listed in the table (c, bottom). Photocurrents are measured in whole-cell voltage-clamp recordings held at −70 mV, with 513 nm light at 1.0 mW mm−2 irradiance. Data are mean and s.e.m.; n = 9 for WT, 6 for E163Q, 5 for C102A, M105A, C133A, C133R, C153A, E163A and C237A, and 4 for the rest. *P < 0.05, **P < 0.01, one-way ANOVA followed by Dunnett’s test. Reversal potentials are measured with identical light stimulation while cells were held at resting potentials from −95 mV to +15 mV in steps of 10 mV. Data are mean and s.e.m. n = 10 for WT and C237A, 6 for E163A and E163Q, 5 for C102A, M105A, C133A and C153A, and 4 for the rest. **P = 0.0022, one-way ANOVA followed by Dunnett’s test. Spectra measurement was performed in two independent trials, with wild type as a positive control. d, Comparison of fast closing (left) and slow closing (right) coefficients of wild-type and Y72F mutant GtACR1. Data are mean and s.e.m. n = 10 for WT and 5 for Y72F. P = 0.7 for both graphs, two-tailed t-test.
