Extended Data Fig. 3: Structure-based sequence alignment of microbial opsin genes.
From: Crystal structure of the natural anion-conducting channelrhodopsin GtACR1
The sequences are GtACR1 (GenBank accession AKN63094.1), GtACR2 (GenBank AKN63095.1), ZipACR (GenBank APZ76709.1), PsuACR1 (GenBank ID: KF992074.1), the chimaeric channelrhodopsin between CrChR1 and CrChR2 (C1C2, PDB code 3UG9)34, CrChR1 (GenBank 15811379), CrChR2 (GenBank 158280944), ChR1 from Volvox carteri (VcChR1, UniProtKB B4Y103), ChR1 from V. carteri (VcChR2, UniProtKB ID: B4Y105), Chrimson (GenBank ID: AHH02126.1), ChR from Tetraselmis striata (TsChR, GenBank ID: KF992089.1), HsBR (PDB code 1C3W)59, HsHR (PDB code 1E12)48, and Krokinobacter eikastus rhodopsin 2 (KR2, PDB code 3X3B)60. The sequence alignment was created using PROMALS3D61 and ESPript 362 servers. Secondary structure elements for GtACR1 are shown as coils and arrows. ‘TT’ represents turns. Cysteine residues forming intermolecular and intramolecular disulfide bridges are highlighted in green and yellow, respectively. The residues of retinal-binding pockets are coloured pink. The residues in the Schiff base region are coloured cyan. The residues forming the ECS2 and CCS are coloured orange and blue, respectively.
