Extended Data Fig. 6: Conformational preferences of non-neutralizing antibodies for HIV-1 Env on virus.
From: Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET
a, b, FRET histograms and overlaid landscapes of HIV-1NL4-3 in the presence of 100 μg ml−1 17b (a) and 100 μg ml−1 F105 (b) acquired after 0 min, 30 min and 60 min of incubation. c, d, FRET histograms and overlaid landscapes of HIV-1BG505 in the presence of 17b (c) and F105 (d), acquired after 0 min, 30 min and 60 min of incubation. Non-neutralizing antibodies have preference for the state 3 conformation of Env. Note that in contrast to the tier 1 HIV-1 isolate NL4-3, the tier 2 isolate BG505 does not respond to 17b. FRET population histograms represent mean ± s.e.m., determined from three independent groups of smFRET traces.
