Extended Data Fig. 5: Artificial and natural modulation of sincRNA levels.
From: Nucleolar RNA polymerase II drives ribosome biogenesis
a, In HEK293T cells treated with the Pol II inhibitor FP, introduction of ASOs targeting sincRNAs lowers IGS ncRNA levels relative to ASO control-treated cells (CTL). ASO-dependent percentage decreases in sincRNA levels are indicated for each IGS site; the average decrease in total sincRNA levels is 49%. Data are means ± s.d.; two-tailed t-test, n = 3 biologically independent experiments. b–d, In the absence of heat shock, artificial overexpression of sincRNA22 (psincRNA) in nucleolar-stress hyperresponsive MCF7 cells failed to repress rRNA biogenesis (b) or rRNA levels (c), despite the enrichment of sincRNA22 in the nucleolar fraction (d). Plasmid (pCTL), iPol I (LAD), vehicle (DMSO) and GAPDH cell fractionation controls were included. Data are means ± s.d.; n = 2 biologically independent experiments (b, d); two-tailed t-test, n = 3 biologically independent experiments (c); e, Quantification of the number of distinct NPM foci per cell in different cell types. Data are means ± s.d.; one-way ANOVA with Tukey’s multiple comparisons test, n = 5 biologically independent experiments.
