Extended Data Fig. 5: Supplementary FACS gating.

a, Representative FACS gating of live EpCAM⁻CD45⁻CD31⁻PDPN⁺PDGFRα⁺ fibroblasts in Figs. 2, 3, Extended Data Figs. 8, 9. b, c, Representative FACS gating on LY6C⁺SCA1⁺ (red), LY6C⁺SCA1⁻ (blue) and LY6C⁻SCA1⁻ (green) PDGFRα⁺ cells (as gated in Extended Data Fig. 7a) in lymph node of Ccl19^YFP mice (b) and lymph node of Grem1^creERT2Rosa26^LSLYFP mice (c). Grem1^creERT2Rosa26^LSLYFP mice received tamoxifen via intraperitoneal injection for five consecutive days and were analysed on days 14–16 after first injection. d, Representative FACS gating on LY6C⁺SCA1⁺ (red), LY6C⁺SCA1⁻ (blue) and LY6C⁻SCA1⁻ (green) PDGFRα⁺ cells (as gated in Extended Data Fig. 7a) in tissues from Dpt^IRESCreERT2Rosa26^LSLYFP mice. Mice received tamoxifen chow for 14 days and were analysed on days 14–16. n = representative of 3 or more biologically independent experiments.