Extended Data Fig. 10: Binding interface between VP35 and EBOV L and its comparison with other nsNSV polymerase complexes.

(a) The binding footprints of VP35 protomers on EBOV L are indicated by black dash line. The interacting residues of L are colored and labelled according to the bound VP35 protomers, and the overlapping regions are shown in orange (VP35b/d) and pink (VP35c/d), respectively. (b) Comparison of the binding interfaces of EBOV L-VP35 and RSV L-P polymerase complexes. The L and P of RSV are colored in black and white, respectively. The L and VP35 of EBOV are colored as in Fig. 4. (c) Sequence alignment of critical interactive residues from L proteins among Mononegavirales. The L residues that formed hydrogen bonds with the residues from VP35 of EBOV and P of RSV are indicated by red and blue stars, respectively.